Cloned (Comment) | Organism |
---|---|
gene bdh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Escherichia coli | Bacillus licheniformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoin + NADH + H+ | Bacillus licheniformis | racemic mixture of (3S/3R)-acetoin | meso-2,3-butanediol + NAD+ | - |
r | |
acetoin + NADH + H+ | Bacillus licheniformis 10-1-A | racemic mixture of (3S/3R)-acetoin | meso-2,3-butanediol + NAD+ | - |
r | |
additional information | Bacillus licheniformis | stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A | ? | - |
? | |
additional information | Bacillus licheniformis 10-1-A | stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | X5F726 | isolated from soil samples | - |
Bacillus licheniformis 10-1-A | X5F726 | isolated from soil samples | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimization of culture conditions or strain 10-1-A and production of enzyme meso-2,3-BDH, overview | Bacillus licheniformis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R)-acetoin + NADH + H+ | - |
Bacillus licheniformis | meso-2,3-butanediol + NAD+ | - |
r | |
(3R)-acetoin + NADH + H+ | - |
Bacillus licheniformis 10-1-A | meso-2,3-butanediol + NAD+ | - |
r | |
acetoin + NADH + H+ | racemic mixture of (3S/3R)-acetoin | Bacillus licheniformis | meso-2,3-butanediol + NAD+ | - |
r | |
acetoin + NADH + H+ | racemic mixture of (3S/3R)-acetoin | Bacillus licheniformis 10-1-A | meso-2,3-butanediol + NAD+ | - |
r | |
additional information | stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A | Bacillus licheniformis | ? | - |
? | |
additional information | stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A | Bacillus licheniformis 10-1-A | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BDH | - |
Bacillus licheniformis |
meso-BDH | - |
Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus licheniformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus licheniformis | |
NADH | - |
Bacillus licheniformis |
General Information | Comment | Organism |
---|---|---|
physiological function | 2,3-butanediol dehydrogenase (BDH) catalyzes the interconversion between acetoin and 2,3-butanediol and is a key enzyme for 2,3-butanediol production | Bacillus licheniformis |